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Alaska Department of Fish and Game


Gene Conservation Laboratory
Laboratory Techniques

The laboratory primarily performs Single Nucleotide Polymorphism (SNP) analyses using genotyping microarray chips (Fluidigm® 96.96 Dynamic Arrays). These chips run 9216 separate multiplex reactions in an internal nanofluidic chamber matrix. 96 genetic samples react against 96 SNP assays. We also perform microsatellite analyses and sequencing using an ABI 3730 capillary sequencer. All processes begin with sample collection, DNA purification, and PCR amplification of the genetic markers.

During the fishing season the laboratory can perform in season analyses of fisheries across the state for stock identification studies. Turnaround time is typically 48 hours to aid managers on a real time basis. The information we provide informs managers and fishermen on the locations of where the mixed ocean salmon will return.

Historically, the laboratory was also equipped to perform allozyme electrophoresis mitochondrial RFLP analysis, and flow cytometry. Allozyme analysis is no longer utilized in the lab and the flow cytometry functions have been transferred to Fort Richardson Hatchery for triploid certification.

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