Gene Conservation Laboratory
Sequencing DNA

Analysis of sequencing DNA is also performed on the ABI 3730 Capillary Sequencer. The 24 horizontal lanes in this photo of a real-time read represent a single fish migrating through a single capillary. The bottom 24 are the reverse complement of the top fish. ABI BigDye® v3.1 Cycle Sequencing chemistry allows for detection of the four nucleotides. Preparing sequencing samples to run on the 3730 is the same process as Microsatellites with one additional step immediately after PCR amplification.

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