Fisheries, Subsistence, and Habitat
Publications Searchable Database
Search Again| Division: | Sport Fish |
| Title: | Development of microsatellite genetic markers for Kenai River Chinook salmon |
| Author: | Begich R. N., W. D. Templin, A. W. Barclay, and L. W. Seeb |
| Year: | 2010 |
| Report ID: | Alaska Department of Fish and Game, Fishery Data Series No. 10-38, Anchorage |
| Abstract: | Significant genetic variation exists among populations of Chinook salmon from Kenai River drainage. Recent analyses using microsatellite markers confirm the results of the earlier studies which detected differences between early- and late-run Chinook salmon based on allozyme and mitochondrial DNA markers. This report presents the results of a survey of 13 microsatellite loci (from a standardized set used by the Pacific Salmon Commission Chinook Technical Committee) in 977 individual fish representing nine Chinook salmon populations in the drainage. Average genotyping failure rate was approximately 4.5%, with the majority of failures in the Slikok Creek collection (success rate ~ 35.9%) where samples mainly came from carcass sampling. Estimates of per-locus FST ranged from 0.019 to 0.045 suggesting a level of divergence among collections that should be useful for management applications. The populations in the baseline could be separated into four groups based on geographic, behavioral, and genetic characteristics: Lower Kenai River tributaries, Kenai River mainstem, Killey River, and Quartz Creek. Simulation results indicate that contributions from these groups can be detected in fishery harvests with a high degree of precision and accuracy (mean correct allocation = 96.7%). |
| Keywords: | Chinook salmon, Oncorhynchus tshawytscha, Cook Inlet, Kenai River, microsatellite |